2024

Takahiro Watanabe, Priyanka Swaminathan, Linnea Björk, Ayaka Nakanishi, Hisako Sato, Tamotsu Zako*, K. Peter R. Nilsson and Mikael Lindgren*
"Spectroscopic Response of Chiral Proteophenes Binding to Two Chiral Insulin Amyloids"
ChemPhotoChem, e202400225 (2024)
[愛媛大プレスリリース][理学部HP]

Yu Muto1, Gen Hirao1 and Tamotsu Zako* (1 equal contribution)
"Detection of estradiol with a digital immunoassay using an anti-immunocomplex antibody and single-molecule observation of gold nanoparticles"
Analytical Sciences, 40, 975-979 (2024)
[Hot Article Award][愛媛大プレスリリース][理学部HP]

Yusuke Tsuchie1, Soichiro Kusuda1, Haruka Kawabe, Wakako Mori, Mikael Lindgren, Yutaka Watanabe, Tamotsu Zako* (1, equal contribution)
"Polyallylamine binds to Aβ amyloid and inhibits antibody recognition"
International Journal of Molecular Sciences, 25, 3112(12p) (2024)


Youji Shimazaki, Shinya Enomoto and Saki Ishiko
"Separation of lysozyme-ovotransferrin complexes and the cooperative role of their components in egg white"
Applied Biochemistry and Biotechnology in press (2024)

島崎洋次
「非変性条件の2次元電気泳動による各種酵素およびその複合体の分離と機能解析」[第62回日本電気泳動学会学会賞（児玉賞）受賞者論文]
電気泳動, in press

2023

Gen Hirao, Nanami Fukuzumi, Atsushi Ogawa, Tsuyoshi Asahi, Mizuo Maeda and Tamotsu Zako*
"Effect of DNA density immobilized on gold nanoparticles on nucleic acid detection"
RSC Advances, 13, 30690-30695 (2023)
[愛媛大プレスリリース][理学部HP]

Haruka Kawabe, Satoshi Ikawa, Katsuhisa Kitano and Tamotsu Zako*
"Peroxynitric acid inhibits amyloid β aggregation"
Biochemical and Biophysical Research Communications, 660, 1-5 (2023)

Kenichiro Nagata, Ryo Ashikaga, Wakako Mori, Tamotsu Zako and Youji Shiazaki*
"Analysis of the enzymatic degradation of lysozyme fibrils using a combination method of non-denaturing gel electrophoresis and double staining with Coomassie Brilliant Blue G-250 and R-250 dyes"
Analytical Sciences, 39, 267-274 (2023)

Maho Inoue and Youji Shimazaki
"Applicability of esterase activity change analysis using a combined method of non-denaturing two-dimensional electrophoresis and reverse staining"
Journal of Electrophoresis, 67, 1-7 (2023)

島崎洋次, 福家麗, 徳田深咲
「非変性2次元ゲル電気泳動法によるネイティブタンパク質の分離分析法の展望と課題」
電気泳動 67, 71-75 (2023)


永瀬 晃正, 岩屋 啓一, 座古 保, 菊地 実, 桂 善也
「インスリン由来アミロイドーシス（インスリンボール）とインスリン療法の皮膚合併症」
日本薬理学雑誌、158, 173-177 (2023)

島崎洋次, 中尾香琳, 福家麗
「非変性2次元電気泳動法によって分離·検出された カルボキシルエステラーゼとトランスフェリン複合体の電気泳動的抽出」(総合論文）
電気泳動 67, 37-42 (2023)

2022

Naoe Kaneko1, Wakako Mori1, Mie Kurata, Toshihiro Yamamoto, Tamotsu Zako and Junya Masumoto*(1, equal contribution)
"Inflammasome assembly is required for intracellular formation of β2-microglobulin amyloid fibrils, leading to IL-1β secretion"
International Journal of Immunopathology and Pharmacology, 36, 1-15 (2022)

Wakako Mori, Ryosuke Kawakami, Yosuke Niko, Tomohiro Haruta, Takeshi Imamura, Kentaro Shiraki and Tamotsu Zako*
"Differences in interaction lead to the formation of different types of insulin amyloid"
Scientific Reports, 12, 8556 (2022)
[愛媛大プレスリリース][理学部HP]

Yu Muto and Tamotsu Zako*
"Colorimetric detection of thrombin based on signal amplification by transcription-reverse transcription concerted reaction using non-crosslinking aggregation of gold nanoparticles"
Analytical Sciences, 38, 3-7 (2022)
[Hot Articles]

Katsutoshi Hori * , Shogo Yoshimoto , Tomoko Yoshino , Tamotsu Zako , Gen Hirao , Satoshi Fujita, Chikashi Nakamura , Ayana Yamagishi and Noriho Kamiya
"Recent advances in research on biointerfaces: from cell surfaces to artifical interfaces"
Journal of Bioscience and Bioengineering, 133, 195-207 (2022)

Karin Nakao and Youji Shimazaki*
"Electrophoretic extraction of protein complexes after separation and detection by a combined method of non-denaturing two-dimensional electrophoresis and reversible staining"
Journal of Electrophoresis, 66, 5-11 (2022)


平尾元、座古保
「DNA密生金ナノ粒子で分子を検出！――環境中の微量なホルモン分子の簡易検出法へ道を拓く」
化学, 77, 47-51 (2022)

2021

武藤悠、座古保*
「金ナノ粒子を用いるカロリメトリックバイオセンサー」
分析化学, 70, 661-670 (2021)

Risako Kunitomi, Ambara R. Pradipta, Haruka Kawabe, Nadine Lobsiger, Katsunori Tanaka and Tamotsu Zako*
"Inhibition of amyloid formation of amyloid β (1-42), amylin and insulin by 1,5-diazacyclooctanes, a spermine-acrolein conjugate"
Bioorganic & Medicinal Chemistry, 46, 116391 (2021)

Wakako Mori1, Naoe Kaneko1, Ayaka Nakanishi, Tamotsu Zako and Junya Masumoto* (1, equal contribution)
"Insulin amyloid fibrils interact directly with the NLRP3, resulting in inflammasome activation and pyroptotic cell death"
International Journal of Immunopathology and Pharmacology, 35, 1-7 (2021)

Yu Muto, Gen Hirao and Tamotsu Zako*
"Transcription-Based Amplified Colorimetric Thrombin Sensor Using Non-Crosslinking Aggregation of DNA-Modified Gold Nanoparticles "
Sensors (Basel), 21, 4318 (11p) (2021)

Yuki Yano-Ozawa, Nadine Lobsiger, Yu Muto, Takahiro Mori, Ken Yoshimura, Yuki Yano, Wendelin Jan Stark, Mizuo Maeda, Tsuyoshi Asahi, Atsushi Ogawa and Tamotsu Zako*
"Molecular detection using aptamer-modified gold nanoparticles with an immobilized DNA brush for the prevention of non-specific aggregation"
RSC Advances, 11, 11984-11991 (2021)
[愛媛大プレスリリース][理学部HP]

Wakako Mori, Keisuke Yuzu, Nadine Lobsiger, Hideo Nishioka, Hisako Sato, Terumasa Nagase, Keiichi Iwaya, Mikael Lindgren and Tamotsu Zako*
"Degradation of Insulin Amyloid by Antibiotic Minocycline and Formation of Toxic Intermediates"
Scientific Reports, 11, ID: 6857 (2021)
[愛媛大プレスリリース][理学部HP]

Ken Yoshimura, Patmawati, Mizuo Maeda, Noriho Kamiya and Tamotsu Zako*
"Protein-functionalized gold nanoparticles for antibody detection using the darkfield microscopic observation of nanoparticle aggregation"
Analytical Sciences, 37, 507-511 (2021)

Youji Shimazaki* and Shunta Yabu
"Characterization of enzymatic activity of lysozyme in lysozyme–ovotransferrin complex before and after treatment with trypsin"
Separation Science Plus, 4, 377-383 (2021)


平尾元、座古保
「金ナノ粒子凝集体の一分子計測による高感度分子検出」
（「金属ナノ粒子、微粒子の合成、調整と最新応用技術」（第７章・第２節））
（技術情報協会、2021年10月）

2020

Keisuke Yuzu, Mikael Lindgren, Sofie Nyström, Jun Zhang, Wakako Mori, Risako Kunitomi, Terumasa Nagase, Keiichi Iwaya, Per Hammarström and Tamotsu Zako*
"Insulin Amyloid Polymorphs: Implications for Iatrogenic Cytotoxicity"
RSC Advances, 10, 37721-37727 (2020)
[愛媛大プレスリリース][理学部HP]

Terumasa Nagase*, Keiichi Iwaya, Koichiro Kogure, Tamotsu Zako, Yohei Misumi, Minoru Kikuchi, Koichi Matsumoto, Masayuki Noritake, Yasuhiro Kawachi, Masaki Kobayashi, Yukio Ando and Yoshiya Katsura
"Insulin‐derived amyloidosis without a palpable mass at the insulin injection site: A report of two cases"
Journal of Diabetes Investigation, 11, 1002-1005 (2020)

Youji Shimazaki*, Aoshi Inoue and Haruka Ikeuchi
"Electrophoretic injection and reaction of dye-bound enzymes to protein and bacteria within gel"
Journal of Microbiol Methods, 176, 106208 (2020)

Youji Shimazaki* and Hikari Nakamaru
"Production of Dye-binding Esterase Reactor after Separation and Detection Using Combined Native Isoelectric Focusing and Blue Native Electrophoresis"
Journal of Electrophoresis, 64, 1-6 (2020)

2019

Masaru Oda*, Kosuke Sasano, Akira Nishi, Tamotsu Zako and Toshiro Tani
“Gel electrophoresis and hybridazation of DNA-functionalized quantum-dots as part of a study of linear quantum dots chains”
Journal of Physics: Conference Series, 1220, 012051 (2019)

Keiichi Iwaya1, Tamotsu Zako1, Junta Fukunaga, Karin Margareta Sorgjerd, Kentaro Ogata, Koichiro Kogure, Hiroshi Kusano, Masayuki Noritake, Mizuo Maeda, Yukio Ando, Yoshiya Katsura and Terumasa Nagase* (1, equal contribution)
“Toxicity of insulin-derived amyloidosis:a case report”
BMC Endocrine Disorders, 19, Article number: 61 (2019)

Yovita Djohan1, Tomoki Azukizawa1, Patmawati, Kotaro Sakai, Fumiya Sato, Ryoji Takahashi, Masafumi Yohda, Mizuo Maeda, Noriho Kamiya and Tamotsu Zako* (1, equal contribution)
“Molecular chaperone assisted-biosynthesis of gold nanoparticles with improved size distribution and dispersion”
Biomaterials Science, 7, 1801-4 (2019)

Yuki Yano, Masamichi Nisougi, Yuki Yano-Ozawa, Tsuyoshi Ohguni, Atsushi Ogawa, Mizuo Maeda, Tsuyoshi Asahi and Tamotsu Zako*
“Detection of Gold Nanoparticles Aggregation using Light Scattering for Molecular Sensing”
Analytical Sciences, 35, 685-690 (2019)
[Hot Articles]

Risa Kawano, Ayaka Nakanishi, Tamotsu Zako and Youji Shimazaki*
“Analysis of the degradation of amyloid beta fibrils after separation via the combination of non-denaturing agarose electrophoresis and Congo red dye staining"
Separation Science Plus, 2, 322-328 (2019)
[Journal Cover]


Youji Shimazaki* and Suzuka Inoue
“Removal of Copper in Microdroplets Ovomucoid Hydrolystaes Bound to Reverse-Phase Chromatography Media Within Pipette Tips”
Applied Biochemistry and Biotechnology, 189, 680-689 (2019)

2018

Ayaka Nakanishi, Naoe Kaneko, Hiroyuki Takeda, Tatsuya Sawasaki, Shinnnosuke Morikawa, Wei Zhou, Mie Kurata, Toshihiro Yamamoto, Sheikh Mohammad Fazle Akbar, Tamotsu Zako and Junya Masumoto*
“Amyloid beta directly interacts with NLRP3 to initiate inflammasome activation: identification of an intrinsic NLRP3 ligand in a cell-free system”
Inflammation and Regeneration, 38, 27 (8p) (2018)

Shinnosuke Morikawa, Naoe Kaneko Chikara Okumura, Haruka Taguchi, Mie Kurata, Toshihiko Yamamoto, Haruhiko Osawa, Ayaka Nakanishi, Tamotsu Zako and Junya Masumoto*
“IAPP/amylin deposition, which is correlated with espression of ASC and IL-1b in beta-cells of Langerhans’ islets, directly initiates NLRP3 inflammasome activation”
International Journal of Immunopathology and Pharmacology, 32, 1-10 (2018)

Jinhua Dong, Richi Fujita, Tamotsu Zako and Hiroshi Ueda*
“Construction of Quenchbodies to detect and image amyloid-beta oligomers”
Analytical Biochemistry, 550, 61-67 (2018)

Muhamad Sahlan, Tamotsu Zako and Masafumi Yohda*
“Prefoldin, a jellyfish-like molecular chaperone: Functional cooperation with a group II chaperonin and beyond”
Biophysical Reviews, 10, 339-45 (2018)

Youji Shimazaki* and Ayumi Takahashi
“Antibacterial activity of lysozyme-binding proteins from chicken egg white"
Journal of Microbiological Methods 154, 19-24 (2018)

Youji Shimazaki*, Yoshiko Ochi and Kennosuke Fujimura
Microscale isolation of native forms of lysozyme from chicken egg white by gel isoelectric focusing.
Electrophoresis, 39, 1054-1061 (2018)

Youji Shimazaki*, Kosuke Tanaka and Keisuke Sakata
"Enzyme inhibitory activity of ovomucoid extracted using a carboxypeptidase Y-immobilized membrane"
Chromatography, 39, 33-9 (2018)

2017

Guoqing Wang, Tong Bu, Tamotsu Zako*, Ryoko Watanabe-Tamaki, Takuo Tanaka, Mizuo Maeda*
"Dark field microscopic analysis of discrete Au nanostructures: Understanding the correlation of scattering with stoichiometry"
Chemical Physics Letters, 684, 310-5 (2017)

Youji Shimazaki*, Rino Miyatsuka
"Hydrolytic Activity of Esterase-Antibody Complexes Retained Within Gel Capsules After Complex Isolation"
Applied Biochemistry and Biotechnology, 182, 1208-17 (2017)

2016

Hiroki Jozawa, Md. Golam Kabir, Tamotsu Zako, Mizuo Maeda, Chiba Kazuhiro, Yutaka Kuroda*
"Amorphous protein aggregation monitored using fluorescence self‐quenching"
FEBS Letters, 590, 3501-9 (2016)

Ayumi Tsutsui#, Tamotsu Zako#, Tong Bu, Yoshiki Yamaguchi, Mizuo Maeda and Katsunori Tanaka*,
"1,5-Diazacyclooctanes, as Exclusive Oxidative Polyamine Metabolites, Inhibit Amyloid-β(1-40) Fibrillization"
Advanced Science, 3, 1600082 (5 pages) (2016)
#: equal contribution
[Journal Cover] [理研プレスリリース] [RIKEN Press Release ENG] [愛媛大 HP] [愛媛新聞] [日経電子版] [薬事日報]

Tamotsu Zako*, Masaaki Ito*, Hiroshi Hyodo, Miya Yoshimoto, Masayuki Watanabe, Hiroshi Takemura, Hidehiro Kishimoto, Kazuhiro Kaneko, Kohei Soga* and Mizuo Maeda
"Extra-luminal detection of assumed colonic tumor site by near-infrared laparoscopy"
Surgical Endoscopy, 30, 4153-4159 (2016)

Tamotsu Zako, Muhamad Sahlan, Sayaka Fujii, Yohei Y. Yamamoto, Phan The Tai, Kotaro Sakai, Mizuo Maeda and Masafumi Yohda*
"Contribution of the C-terminal region of a group II chaperonin to its interaction with prefoldin and substrate transfer"
J. Mol. Biol., 428, 2405-2417 (2016)

Tong Bu, Tamotsu Zako* and Mizuo Maeda
"Dark field microscopic sensitive detection of amyloid fibrils using gold nanoparticles modified with antibody"
Anal. Sci, 32, 307-311 (2016)
[愛媛新聞（関連記事）]

Masaki Saito, Tamotsu Zako, Ryoji Takahashi and Youj Shimazaki*
"Inhibition of amyloid β protein fibrillation via carboxypeptidase Y after protein trapping using immunoaffinity membranes"
Chemistry Letters, 45, 1241-1243 (2016)

Youji Shimazaki* and Yuki Sato
"Retaining activity of enzymes after capture and extraction within a single-drop of biological fluid using immunoaffinity membranes" 
J. Chromatography B, 1021, 108-113 (2016)

2015

Tong Bu, Tamotsu Zako*, Martin Zeltner, Karin M. Sorgjerd, Christoph M. Schumacher, Corinne J. Hofer, Wendelin J. Stark* and Mizuo Maeda*
"Adsorption and separation of amyloid beta aggregates using ferromagnetic nanoparticles coated with charged polymer brushes"
J. Mater. Chem. B, 3, 3351-3357 (2015)

Toshiaki Arai, Shinya Kimata, Daichi Mochizuki, Keita Hara, Tamotsu Zako, Masafumi Odaka, Masafumi Yohda,
Fumio Arisaka, Shuji Kanamaru, Takashi Matsumoto, Shunsuke Yajima, Junichi Sato, Shinji Kawasaki, and Youichi Niimura*
"NADH oxidase and alkyl hydroperoxide reductase subunit C (peroxiredoxin) from Amphibacillus xylanus form an oligomeric assembly"
FEBS Open Bio 5, 124-131 (2015)

Tamotsu Zako*, Miya Yoshimoto, Hiroshi Hyodo, Hidehiro Kishimoto, Masaaki Ito, Kazuhiro Kaneko, Kohei Soga* and Mizuo Maeda
"Cancer-targeted Near Infrared imaging using rare earth ion-doped ceramic nanoparticles"
Biomater. Sci. 3, 59-64 (2015)
selected as inside front cover



Youji Shimazaki* and Yoko Takatsu,
"Combined Method of Immunoaffinity Membrane Within Tubes and MALDI-TOF MS for Capturing and Analyzing Amyloid Beta"
Appl. Biochem. Biotechnol. 177, 1565-1571 (2015)

2014

Tamotsu Zako* and Mizuo Maeda
"Application of biomaterials for the detection of amyloid aggregates" (minireview)
Biomater. Sci. 2, 951-955 (2014)

Masayuki Watanabe, Hiroshi Takemura, Hiroshi Mizoguchi, Hiroshi Hyodo, Kohei Soga, Tamotsu Zako, Hidehiro Kishimoto, Masaaki Ito, Kazuhiro Kaneko
"Development of Novel Endoscope with NIR Camera Using Real-time Video Composite Method"
The 15th International Conference on Biomedical Engineering. IFMBE Proceedings, vol 43, 128-131


Eisuke Takai, Ken Uda, Shuhei Matsushita, Yui Shikiya, Yoichi Yamada, Tamotsu Zako, Mizuo Maeda and Kentaro Shiraki*
"Cysteine Inhibits Amyloid Fibrillation of the Lysozyme and Directs the Formation of Small Worm-like Aggregates through Non-Covalent Interactions"
Biotech. Prog. 30, 470-478 (2014)

Eisuke Takai, Gai Ohashi, Tomonori Yoshida, Karin M. Sorgjerd, Tamotsu Zako, Mizuo Maeda, Katsuhisa Kitano and Kentaro Shiraki*
"Degeneration of Amyloid-b Fibrils by Low Temperature Atmospheric Pressure Plasma in Aqueous Solution"
Appl. Phys. Lett. 104, 023701 (2014)

Eisuke Takai, Ken Uda, Tomonori Yoshida, Tamotsu Zako*, Mizuo Maeda, Yoichi Yamada and Kentaro Shiraki*
"Cysteine Inhibit Fibrillization and Cytotoxicity of Amyloid-β 40 and 42: Implication for Contribution of Thiophilic Interaction"
Phys. Chem. Chem. Phys. 16, 3566-3572 (2014)

Youji Shimazaki*, Ai.Hashimoto
"A microfluidic device containing membrane-immobilized antibodies forsuccessively capturing cytosolic enzymes"
Talanta.125, 400-404 (2014).

Ayaka Kimura, Youji Shimazaki*
"Micro-scale extraction and analysis of intactcarboxylesterase after trapping on an immunoaffinity membrane surface"
Applied Biochemistry and Biotechnology 172, 4053-4061 (2014)

2013

Aline C.C. Rotzetter, Christoph M. Schumacher, Tamotsu Zako*, Wendelin J. Stark* and Mizuo Maeda
"Rapid Surface- Biostructure Interaction Analysis using strong Metal-based Nanomagnets"
Langmuir 29, 14117-14123 (2013)

Helene Vignaud, Claude Bobo, Ioan Lascu, Karin M. Sorgjerd, Tamotsu Zako, Mizuo Maeda, Benedicte Salin, Sophie Lecomte and Christophe Cullin*
"A structure-toxicity study of Abeta42 reveals a new anti-parallel aggregation pathway"
PLoS ONE 8,e80262 (2013)

Tong Bu, Tamotsu Zako*, Masahiro Fujita and Mizuo Maeda*
"Detection of DNA Induced Gold Nanoparticle Aggregation with Dark Field Imaging"
Chem. Commun. 49, 7531-7533 (2013)

Erika Tashiro, Tamotsu Zako, Hideki Muto, Yoshinori Itoo, Karin Sorgjerd, Naofumi Terada, Akira Abe, Makoto Miyazawa, Akira Kitamura, Hirotake Kitaura, Hiroshi Kubota, Mizuo Maeda, Takashi Momoi, Sanae M.M. Iguchi-Ariga, Masataka Kinjo, and Hiroyoshi Ariga*
"Prefoldin protects neuronal cells from polyglutamine toxicity by preventing aggregation formation"
J. Biol. Chem. 288, 19958-19972 (2013)

Karin M. Sorgjerd, Tamotsu Zako*, Masafumi Sakono, Peter C Stirling, Michel R Leroux, Takashi Saito, Per Nilsson, Misaki Sekimoto, Takaomi C. Saido, and Mizuo Maeda*
"Human prefoldin inhibits Abeta fibrillation and contributes to formation of non-toxic Abeta aggregates"
Biochemistry 52, 3532-3542 (2013)

RIKEN press release (in Japanese)
Highlighted in RIKEN Research (in English)

Naofumi Terada, Tamotsu Zako* and Mizuo Maeda
"Photon counting histogram using numerical data of point spread function"
Jpn. J. Appl. Phys. 53, 038001 (2013)

Masafumi Sakono, Arata Utsumi, Tamotsu Zako*, Tetsuya Abe, Masafumi Yohda and Mizuo Maeda
"Formation of non-toxic Aβ fibrils by small heat shock protein under heat-stress condition"
Biochem. Biophys. Res. Commun. 430, 1259-64 (2013)

Youji Shimazaki*, Madoka Michhiro
"Analysis of trypsininhibition activity in human plasma proteins after separation by non-denaturingtwo-dimensional electrophoresis"
Clinica Chimica Acta 425, 48-53 (2013).

Youji Shimazaki*,YuriNishimura, and Masaki Saito
"Antigen digestion on the target plate ofMALDI-TOF MS after isolation using an immunoaffinity membrane"
Journal of Pharmaceutical and Biomedical Analysis 83, 293-298 (2013).

Youji Shimazaki*, Shizuka Miki
"Analysis of enzyme activity regulation by non-denaturingelectrophoresis and application of this regulation for enzyme reactor production"
Journal of Enzyme Inhibitionand Medical Chemistry 28, 894-899 (2013)

2012

Ryoko Watanabe-Tamaki, Atsushi Ishikawa, Takuo Tanaka*, Tamotsu Zako and Mizuo Maeda
"DNA-Templating Mass Production of Gold Trimer Rings for Optical Metamaterials"
J. Phys. Chem. C, 116, 15028-15033 (2012)

Katarzyna Maria Psonka-antonczyk, Julien Duboisset, Bjorn Torger Stokke, Tamotsu Zako, Takahiro Kobayashi,
Mizuo Maeda, Sofie Nystrom, Jeff Mason, Per Hammarstrom, K. Peter. R. Nilsson, Mikael Lindgren*
"Nanoscopic and photonic ultrastructural characterization of two distinct insulin amyloid states"
Int. J. Mol. Sci. 13, 1461-1480 (2012)

Tamotsu Zako*, Masafumi Sakono, Takahiro Kobayashi, Karin Sorgjerd, K. Peter R. Nilsson, Per Hammarstrom, Mikael Lindgren and Mizuo Maeda*,
"Cell interaction study of amyloid using luminescent conjugated polythiophene: Implication that amyloid cytotoxicity is correlated with prolonged cellular binding"
ChemBioChem 13, 358-363 (2012)

Masafumi Sakono*, Tamotsu Zako, Masafumi Yohda and Mizuo Maeda
"Amyloid oligomer detection by immobilized molecular chaperone"
Biochem. Eng. J. 61, 28-33 (2012)

Masafumi Sakono*, Tamotsu Zako and Mizuo Maeda
"Naked-eye Detection of Amyloid Aggregates using Gold Nanoparticles Modified with Amyloid Beta Antibody"
Anal. Sci. 28, 73-76 (2012)

Takahiro Sakikawa , Youji Shimazaki*
"Enzymolysis of high density lipoprotein with acombination of membrane-immobilized esterase and trypsin",
Journal of Pharmaceutical and Biomedical Analysis 71, 179-182 (2012).

Youji Shimazaki*, Yoshinori Kohno, Izumi Fukui, and Toshiharu Koyashiki
"Epitope analysis usingmembrane-immobilized avidin and protein A"
Protein Expression and Purification. 83, 177-181 (2012)

Youji Shimazaki*, Yoshinori Kohno
"Successive analysis of antigen trapping andenzymatic digestion on membrane-immobilized avidin"
Analytical Biochemistry 422, 55-57 (2012)

Youji Shimazaki*, Takahiro Sakikawa, and Ayaka Kimura
"Analysisof activity of esterase captured onto an immunoaffinity membrane"
ClinicaChimica Acta 413, 269-272 (2012)

2011

Eri Takeda, Tomoyuki Murakami, Go Matsuda, Hironobu Murakami, Tamotsu Zako, Mizuo Maeda, Yoko Aida*
"Nuclear Exportin Receptor CAS Regulates the NPI-1-Mediated Nuclear Import of HIV-1 Vpr"
PLoS ONE 6, e27815 (2011)

Masafumi Sakono*, Shigenori Akiyama, Tamotsu Zako*, Shujiro Sakaki, Tomonori Waku, Naoki Tanaka* and Mizuo Maeda
"Immobilized Insulin Amyloid Enhances Cell Adhesion and Proliferation due to Interaction with Fibronectin"
Chem. Lett. 40, 315-317 (2011)

Masafumi Sakono, Shigenori Akiyama, Tamotsu Zako*, Shujiro Sakaki, Tomonori Waku, Naoki Tanaka* and Mizuo Maeda
"Application of two morphologically different fibrillar and filamentous insulin amyloids as a biomaterial for cell culture surfaces"
React. Funct. Polym. 71, 324-328 (2011)

2010

Masafumi Sakono*, Tamotsu Zako, Srdja Drakulic, Jose M Valpuesta, Masafumi Yohda and Mizuo Maeda
"Size-selective recognition of gold nanoparticles by a molecular chaperone"
Chem. Phys. Lett. (2010) 501, 108-112

Takahito Ohshiro*, Tamotsu Zako, Ryoko Wanatabe-Tamaki, Takuo Tanaka and Mizuo Maeda
"A Facile Method Towards Cyclic Assembly of Gold Nanoparticles Using DNA Template Alone"
Chem. Comm. (2010) 46, 6132-6134

Muhamad Sahlan, Taro Kanzaki, Tamotsu Zako, Mizuo Maeda and Masafumi Yohda*
"Analysis of interaction mode between hyperthermophilic archaeal group II chaperonin and prefoldin using a platform of chaperonin oligomers of various subunit arrangements"
Biochimica et Biophysica Acta. - Proteins and Proteomics (2010) 1804, 1810-1816

Muhamad Sahlan, Tamotsu Zako, Phan The Tai, Akashi Ohtaki, Keiichi Noguchi, Mizuo Maeda, Hideyuki Miyatake, Naoshi Dohmae and Masafumi Yohda*
"Thermodynamic Characterization of the Interaction between Prefoldin and Group II Chaperonin"
J. Mol. Biol. (2010) 399, 628-636

Tamotsu Zako*, Hiroshi Hyodo, Kosuke Tsuji, Kimikazu Tokuzen, Hideki Kishimoto, Masaaki Ito, Kazuhiro Kaneko, Mizuo Maeda and Kohei Soga*
"Development of Near Infrared-Fluorescent Nanophosphors and Applications for Cancer Diagnosis and Therapy"
J. Nanomaterials (2010) 2010, 491471

Kazuki Bando, Tamotsu Zako*, Masafumi SAkono, Mizuo Maeda, Takehiko Wada, Masaki Nishijima, Gaku Fukuhara, Cheng Yang, Tadashi Mori, Tamara C. S. Pace, Cornelia Bohne* and Yoshihisa Inoue*
"Bio-Supramolecular Photochirogenesis with Molecular Chaperone: Enantiodifferentiating Photocyclodimerization of 2-Anthracenecarboxylate Mediated by Prefoldin"
Photochemical & Photobiological Sciences (2010) 9, 655-660

Tamotsu Zako*
Amyloid oligomers
FEBS J. (2010) 277, 1347

Masafumi Sakono and Tamotsu Zako*
"Amyloid oligomers: Formation and toxicity of Abeta oligomers" (Review)
FEBS J. (2010) 277, 1348-1358
(organized this minireview series)
FEBS Journal Top Cited Paper Award

Tamotsu Zako*, Shinya Banba, Muhamad Sahlan, Masafumi Sakono, Naofumi Terada, Masafumi Yohda and Mizuo Maeda*
"Hyperthermophilic archaeal prefoldin shows refolding activity at low temperature"
Biochem. Biophys. Res. Commun. (2010) 391, 467-470

~2009

Tamotsu Zako*, Hiroyasu Nagata, Naofumi Terada, Arata Utsumi, Masafumi Sakono, Masafumi Yohda, Hiroshi Ueda, Kohei Soga* and Mizuo Maeda
"Cyclic RGD Peptide-Labeled Upconversion Nanophosphors for Tumor Cell-Targeted Imaging"
Biochem. Biophys. Res. Commun. (2009) 381, 54-58

Tamotsu Zako*, Masafumi Sakono, Naomi Hashimoto, Masaki Ihara and Mizuo Maeda
"Bovine Insulin Filaments Induced by Reducing Disulfide Bonds Show a Different Morphology,
Secondary Structure and Cell Toxicity from Intact Insulin Amyloid Fibrils"
Biophysical J. (2009) 96, 3331-3340

RIKEN press release (in Japanese)
Highlighted in RIKEN RESEARCH

Kazuhiko Hosono, Taro Ueno, Hideki Taguchi, Fumihiro Motojima, Tamotsu Zako, Masasuke Yoshida and Takashi Funatsu*
"Kinetic Analysis of Conformational Changes of GroEL Based on the Fluorescence of Tyrosine 506."
Protein J. (2008) 27,461-8

Masafumi Sakono, Tamotsu Zako*, Hiroshi Ueda, Masafumi Yohda and Mizuo Maeda*
"Formation of highly toxic soluble amyloid beta oligomers by the molecular chaperone prefoldin"
FEBS J. (2008) 275, 5982-5993

selected in the virtual issue on "Protein Misfolding, Prions and Amyloid"

Mihoko Suzuki, Taro Ueno, Ryo Iizuka, Takahiro Miura, Tamotsu Zako, Rena Akahori, Takeo Miyake, Naonobu Shimamoto, Mutsuko Aoki, Takashi Tanii,
Iwao Ohdomari and Takashi Funatsu*
"Effect of the C-terminal truncation on the functional cycle of chaperonin groel:
Implication that the C-terminal region facilitates the transition from the folding-arrested to the folding-competent state."
J. Biol. Chem.(2008) 283, 23931-23939

Tamotsu Zako*, Hiroyasu Nagata, Naofumi Terada, Masafumi Sakono, Kohei Soga,* and Mizuo Maeda
"Improvement of dispersion stability and characterization of upconversion nanophosphors
covalently modified with PEG as a fluorescence bioimaging probe"
J. Materials Sci.(2008) 43, 5325-5330

Tamotsu Zako*, Ryo Iizuka, Taro Kanzaki, Mizuo Maeda and Masafumi Yohda
"Chaperonin and prefoldin - Two molecular chaperones that work cooperatively in archaea and eukaryote" (Review)
in Heat shock proteins :New Research (Edited by Emma Moreland and Camille Vincent),
Nova Science Publishers, Inc., New York, USA ,(2008) pp. 393-416

Eiji Kurimoto, Yohei Nishi, Yoshiki Yamaguchi, Tamotsu Zako, Ryo Iizuka, Naoki Ide, Masafumi Yohda and Koichi Kato
"Dynamics of group II chaperonin and prefoldin probed by 13C NMR spectroscopy"
Proteins (2008) 70,1257-1263

Takeo Miyake, Takashi Tanii, Koichi Kato, Tamotsu Zako, Takashi Funatsu and Iwao Ohdomari
"Selectivity improvement in protein nanopatterning with a hydroxy-terminated self-assembled monolayer template"
Nanotechnology (2007) 18, 305304

Hiromi Horiuchi, Noriya Iwami, Fumi Tachibana, Akashi Ohtaki, Ryo Iizuka, Tamotsu Zako, Masaru Oda, Masafumi Yohda and Toshiro Tani
"Complex formation of CdSe/ZnS/TOPO nanocrystal vs. molecular chaperone in aqueous solution by hydrophobic interaction"
J. Luminescence (2007) 127, 192-197

Tamotsu Zako, Yosuke Murase, Ryo Iizuka, Takao Yoshida, Taro Kanzaki, Naoki Ide, Mizuo Maeda, Takashi Funatsu and Masafumi Yohda*
"Localization of Prefoldin Interaction Sites in the Hyperthermophilic Group II Chaperonin and
Correlations between Binding Rate and Protein Transfer Rate"
J. Mol. Biol. (2006) 364, 110-120

Yoshida, T., Kanzaki, T., Iizuka, R., Komada, T., Tamotsu Zako, Suzuki, R., Maruyama, T. and Yohda, M.*
"Contribution of the C-terminal region to the thermostability of the group II chaperonin
from the hyperthermophilic archaeum, Thermococcus sp. strain KS-1"
Extremophiles (2006) 5, 451-9

Terada, N., Tadakuma H., Ishihama Y., Yamagishi M., Tamotsu Zako and Funatsu T.*
"Analysis of Nuclear Microenvironments by Translational Diffusion of GFP Using Fluorescence Correlation Spectroscopy"
Bioimages (2005) 13, 1-10

Iizuka R., Yoshida T., Ishii N., Tamotsu Zako, Takahashi K., Maki K., Inobe T., Kuwajima K. and Yohda M.*
"Characterization of archeal group II chaperonin-ADP-metal fluoride complexes:
Implications that group II chaperonins operate as a "two-stroke engine."
J. Biol. Chem. (2005) 280,40375-83

Hirose M., Tohda H., Giga-Hama Y., Tsushima R., Tamotsu Zako, Iizuka R., Pack C., Kinjo M., Ishii N. and Yohda M.*
"Interaction of a small heat shock protein of the fission yeast, Schizosaccharomyces pombe,
with a denatured protein at elevated temperature"
J. Biol. Chem. (2005) 280, 32586-93

Ayabe K., Tamotsu Zako and Ueda, H.*
"The role of firefly luciferase C-terminal domain in efficient coupling of adenylation and oxidative steps"
FEBS Lett., (2005) 579, 4389-94

Tamotsu Zako*, Iizuka R., Okochi M., Nomura T., Ueno T., Tadakuma H., Yohda M., Funatsu T.* (* corresponding authors)
"Facilitated release of substrate protein from prefoldin by chaperonin"
FEBS Lett., (2005) 579, 3718-3724

Zhang, G.J.*, Tanii,T., Tamotsu Zako*, Hosaka, T., Miyake,T.，Kanari Y.，Funatsu,T. and Ohdomari, I, (* corresponding authors)
"Nanoscale Patterning of Protein Using Electron Beam Lithography of Organosilane Self-Assembled Monolayers"
Small, (2005) 1, 833-837

Zhang G.J.*, Umezawa H., Hata H., Tamotsu Zako, Funatsu T., Ohdomari I., Kawarada H.
Micropatterning Oligonucleotides on Single Crystal Diamond Surface by Photolithography"
Japanese Journal of Applied Physics, (2005) 44, L295 - L298

Tamotsu Zako*, Funatsu T. and Yohda M.
"Kinetic analysis of interactions between archaeal prefoldin and chaperonin" (Review)
Recent Research Development in Biophysics (2004), 3, 475-483

Tanii T., Hosaka, T., Miyake, T., Zhang, G.J., Tamotsu Zako, Funatsu, T. and Ohdomari, I.
"Preferential immobilization of biomolecules on silicon microstructure array
by means of electron beam lithography on organosilane self-assembled monolayer resist "
Applied Surface Science (2004) 234, 102-106

Okochi M., Nomura T., Tamotsu Zako, Arakawa T., Iizuka R., Ueda H., Funatsu T., Leroux M., Yohda M.*
"Kinetics and binding site for interaction of prefoldin with groupII chaperonin:
contiguous non-native substrate and chaperonin binding sites in archaeal prefoldin"
J Biol Chem. (2004) 279, 31788-31795

Iizuka R., So S., Inobe T., Yoshida T., Tamotsu Zako, Kuwajima K., Yohda M.*
"Role of the helical protrusion in the conformational change and molecular chaperone activity
of the archaeal group II chaperonin. "
J Biol Chem. (2004) 279, 18834-18839

Zhang, G.J.*, Tanii, T., Tamotsu Zako, Funatsu, T. and Ohdomari, I.
"The Immobilization of DNA on Microstructured Patterns Fabricated by Maskless Lithography"
Sensors and Actuators B: Chemical (2004) 97, 243-248

Yamaguchi,S., Mannen, T., Tamotsu Zako, Kamiya, N. and Nagamune, T. *
"Observation of conformational change in immobilized protein by measuring the adsorption amount
of hydrophobic probe with surface plasmon resonance sensor"
Biotechnology Progress (2003) 19, 1348-1354

Tamotsu Zako, Ayabe, K., Aburatani, N., Kamiya, N., Kitayama, A., Ueda, H.* and Nagamune, T.*
"Luminescent and substrate binding activities of firefly luciferase N-terminal domain"
Biochimica et Biophysica Acta., (2003) 1649, 183-189

Fukano, H., Tamotsu Zako, Suzuki, E., Watanabe, K. and Nagamune, T.
"Genetically engineered active Qβ Replicase in rabbit reticulocyte cell-free system
: a fusion protein of EF-Ts is functional as the subunit of Qβ　Replicase."
J. Biosci. and Bioeng.,(2002) 93, p20-24

Yamaguchi, S., Mannen, T., Tamotsu Zako and Nagamune, T.*
"Evaluation of surface hydrophobicity of proteins on solid phase using surface plasmon resonance sensor”
J. Chem. Eng. Jap.,(2001) 27, p191-1964

Tamotsu Zako, Harada, K., Mannen, T., Yamaguchi, S., Kitayama, A., Ueda, H., Nagamune, T.*
"Monitoring the refolding process of immobilized firefly luciferase with a biosensor based on surface plasmon resonance”
J. Biochem.,(2001) 129, p1-43

Tamotsu Zako, Deguchi, H., Kitayama, A., Ueda, H., Nagamune, T.*
Refolding of firefly luciferase immobilized on agarose beads"
J. Biochem.,(2000) 127, p351-3542